The most frequently asked question in current analyses of different microbiomes is what organism(s) differentiates these two groups. As microbiologist and aspiring bioinformaticians we are all aware that due to the complex community structures of microbiomes, and the resulting complex sequence data, finding the appropriate tools to answer this question isn’t straight forward. Accordingly, this week’s human microbiome journal club will look at a paper from Peddada and colleagues detailing their new tool ANCOM (ANalysis Of Composition Of Microbiomes).
In the paper ANCOM is compared to two other potential methods of group comparison, the t-test and the Zero Inflated Gaussian or ZIG method.
Please come out to the journal club as we will aim to
– Better understand how ANCOM works (including the supplement)
– Assess its strength at understanding some of our results
– Learn to implement ANCOM for our uses
Conversation starts at 3pm in HSC 3N10A and will likely continue at the Phoenix afterwards.
Siddhartha Mandal, Will Van Treuren, Richard A. White, Merete Eggesbø, Rob Knight, Shyamal D. Peddada
Microb Ecol Health Dis. 2015; 26: 10.3402/mehd.v26.27663. Published online 2015 May 29. doi: 10.3402/mehd.v26.27663
- PMCID: PMC4450248